Product Name

MYPN-Specific antibody

Documents

Description

MYPN-Specific Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IHC detected in human skeletal muscle tissue, human heart tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 145 kDa

Tested applications

ELISA, WB, IF, IHC

Species reactivity

Human; other species not tested.

Alternative names

145 kDa sarcomeric protein antibody; MYOP antibody; myopalladin antibody; MYPN antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Peptide (Accession Number: NM_032578). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113000(MYPN-Specific antibody) at dilution of 1:300

Immunofluorescent analysis of HepG2 cells using Catalog No:113000(MYPN-Specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:113000(MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:113000(MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens)

Background

MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines (from OMIM). Myopalladin and palladin share C-terminal fragment in sequence. While myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. This antibody is specific to myopalladin.

MYPN-Specific antibody

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