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Product Name
MYOM3 antibody
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Description
MYOM3 Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue. Positive IP detected in mouse skeletal muscle tissue. Positive IHC detected in human skeletal muscle tissue. Observed molecular weight by Western-blot: 162 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ35961 antibody; MYOM3 antibody; Myomesin 3 antibody; Myomesin family member 3 antibody; myomesin family antibody; member 3 antibody; RP11 293P20.1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYOM3 recombinant protein (Accession Number: BC067101). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:112994(MYOM3 antibody) at dilution of 1:500
IP Result of anti-MYOM3 (IP:Catalog No:112994, 4ug; Detection:Catalog No:112994 1:700) with mouse skeletal muscle tissue lysate 3600ug.
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:112994(MYOM3 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:112994(MYOM3 Antibody) at dilution of 1:200 (under 40x lens).
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Background
MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate speed fibers of skeletal muscle. Recently high level of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarker for DMD and other neuromuscular disorders. This antibody recognizes the intact MYOM3 protein (160-160 kDa) as well as MYOM3 fragments (100 kDa and 130 kDa). (26060189)
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References
- Rouillon J, Poupiot J, Zocevic A. Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies. Human molecular genetics. 24(17):4916-32. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"