Human Myocilin/MYOC (His Tag) recombinant protein

Human Myocilin/MYOC (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork.

Myocilin

AI957332; JOAG; GPOA; JOAG1; MYOC; TIGR; MGC159032; GLC1A; MYOCILIN

Q99972

MYOC; GLC1A; TIGR

Human Cells

A DNA sequence encoding the full length of human MYOC (Q99972) (Met 1-Met 504) was fused with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human MYOC consists of 483 amino acids and has a predicted molecular mass of 54.7 kDa. Since most of the MYOC was cleaved at Glu 214, generating a C-terminal fragment of 32 kDa, the purified human MYOC migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE