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Product Name
MYO6 Polyclonal Antibody
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Description
Polyclonal antibody to MYO6
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MYO6 antibody; DFNA22 antibody; DFNB37 antibody; Myo6-007 antibody; Myo6-008 antibody; myosin VI antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1016-1285 of human MYO6 (NP_004990.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - MYO6 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MYO6 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - MYO6 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using MYO6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - MYO6 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using MYO6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells (By similarity).
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