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Product Name
MYO5A Polyclonal Antibody
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Description
Polyclonal antibody to MYO5A
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MYO5A antibody; GS1 antibody; MYH12 antibody; MYO5 antibody; MYR12 antibody; myosin VA antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 950-1150 of human MYO5A (NP_000250.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - MYO5A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MYO5A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - MYO5A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using MYO5A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MYO5A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using MYO5A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MYO5A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate cancer using MYO5A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MYO5A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human rectum using MYO5A antibody at dilution of 1:100 (40x lens).
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Background
Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"