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Product Name
MYO1C Polyclonal Antibody
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Description
Polyclonal antibody to MYO1C
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MYO1C antibody; MMI-beta antibody; MMIb antibody; NMI antibody; myr2 antibody; myosin IC antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 769-1028 of human MYO1C (NP_203693.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - MYO1C Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MYO1C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - MYO1C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using MYO1C antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MYO1C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using MYO1C antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MYO1C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using MYO1C antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MYO1C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using MYO1C antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MYO1C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using MYO1C antibody at dilution of 1:100 (40x lens).
Immunofluorescence - MYO1C Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using MYO1C antibody .
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Background
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. Component of the hair cell's (the sensory cells of the inner ear) adaptation-motor complex. Acts as a mediator of adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells. Binds phosphoinositides and links the actin cytoskeleton to cellular membranes.; Isoform 3 is involved in regulation of transcription. Associated with transcriptional active ribosomal genes. Appears to cooperate with the WICH chromatin-remodeling complex to facilitate transcription. Necessary for the formation of the first phosphodiester bond during transcription initiation (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"