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Product Name
MYO18A antibody
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Description
MYO18A Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 230kd,190kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686L0243 antibody; KIAA0216 antibody; MAJN antibody; MYO18A antibody; Myosin containing a PDZ domain antibody; myosin XVIIIA antibody; MYSPDZ antibody; SPR210 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYO18A recombinant protein (Accession Number: NM_001346767). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:1000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112980(MYO18A antibody) at dilution of 1:6000
IP Result of anti-MYO18A (IP:Catalog No:112980, 3ug; Detection:Catalog No:112980 1:300) with HeLa cells lysate 3000ug.
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Background
MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kD proteins, respectively.Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.
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References
- Sever-Chroneos Z, Krupa A, Davis J. Surfactant protein A (SP-A)-mediated clearance of Staphylococcus aureus involves binding of SP-A to the staphylococcal adhesin eap and the macrophage receptors SP-A receptor 210 and scavenger receptor class A. The Journal of biological chemistry. 286(6):4854-70. 2011.
- Sever-Chroneos Z, Murthy A, Davis J. GM-CSF modulates pulmonary resistance to influenza A infection. Antiviral research. 92(2):319-28. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"