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Product Name
MYL2 antibody
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Description
MYL2 Rabbit Polyclonal antibody. Positive WB detected in rat heart tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human heart tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 18-20 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CMH10 antibody; DKFZp779C0562 antibody; MLC 2 antibody; MLC 2v antibody; MLC2 antibody; MLC2v antibody; MYL2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000432). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:20000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
rat heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112942(MYL2 Antibody) at dilution of 1:5000
IP Result of anti-MYL2 (IP:Catalog No:112942, 4ug; Detection:Catalog No:112942 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:112942(MYL2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:112942(MYL2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
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References
- Ban K, Wile B, Cho KW. Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4. Stem cell reports. 5(6):1239-49. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"