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Product Name
Myh9 antibody
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Description
Myh9 Rabbit Polyclonal antibody. Positive IF detected in Hela. Positive IHC detected in human lung cancer. Positive WB detected in HL-60 cells, human kidney tissue. Observed molecular weight by Western-blot: 224 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human; other species not tested.
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Alternative names
Myh9 antibody; TU72.6 antibody; C80049 antibody; Myhn 1 antibody; D0Jmb2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_022410). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:112938(Myh9 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112938(Myh9 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112938(Myh9 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using Myh9 antibody Catalog No:112938 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Myh9 represents myosin, heavy polypeptide 9, non-muscle
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References
- Mhatre AN, Li Y, Bhatia N, Wang KH, Atkin G, Lalwani AK. Generation and characterization of mice with Myh9 deficiency. Neuromolecular medicine. 9(3):205-15. 2007.
- Zhao B, Qi Z, Li Y, Wang C, Fu W, Chen YG. The non-muscle-myosin-II heavy chain Myh9 mediates colitis-induced epithelium injury by restricting Lgr5+ stem cells. Nature communications. 6:7166. 2015.
- Mhatre AN, Li Y, Atkin G, Maghnouj A, Lalwani AK. Expression of Myh9 in the mammalian cochlea: localization within the stereocilia. Journal of neuroscience research. 84(4):809-18. 2006.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"