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Product Name
MYH7 Polyclonal Antibody
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Description
Polyclonal antibody to MYH7
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
MYH7 antibody; CMD1S antibody; CMH1 antibody; MPD1 antibody; MYHCB antibody; SPMD antibody; SPMM antibody; myosin-7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1766-1935 of human MYH7 (NP_000248.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - MYH7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MYH7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Western blot - MYH7 Polyclonal Antibody
Western blot analysis of extracts of rat heart, using MYH7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - MYH7 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using MYH7 antibody at dilution of 1:100 (40x lens).
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Background
Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"