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Product Name
MYCBP antibody
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Description
MYCBP Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, human kidney tissue, human lung tissue, human placenta tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 12kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AMY 1 antibody; AMY1 antibody; AssOCiate of Myc 1 antibody; c myc binding protein antibody; MYCBP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYCBP recombinant protein (Accession Number: NM_012333). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112915(MYCBP antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:112915(MYCBP antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using MYCBP antibody Catalog No:112915 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
MYC binding protein(MYCBP), encoded by MYCBP gene, binds to N-terminal region of MYC, and then MYC can activate E box-dependent transcription. Upon increasing expression of MYC , MYCBP translocates into the nucleus in the S phase of the cell cycle. MYCBP may involve in spermatogenesis, since it's found to be interacted with AKAP84/149 in the mitochondria in somatic cells and sperm. It highly expressed in heart, placenta, pancreas,skeletal muscle and kidney, but low in lung. This is a rabbit polyclonal antibody raised against full-length MYCBP of human origin.
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