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Product Name
MYC-tag antibody
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Description
MYC-tag Rabbit Polyclonal antibody. Positive WB detected in Recombinant protein. Positive IP detected in Transfected HEK-293 cells. Positive IF detected in Transfected HEK-293 cells, MCF-7 cells.
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Tag
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Alternative names
EQKLISEEDL antibody; MYC tag antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYC-tag recombinant protein (Accession Number: 3XMYC tag). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:4000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
Recombinant protein were subjected to SDS PAGE followed by western blot with Catalog No:117334(MYC-tag antibody) at dilution of 1:4000
IP Result of anti-MYC-tag (IP:Catalog No:117334, 4ug; Detection:Catalog No:117334 1:1000) with Transfected HEK-293 cells lysate 300ug.
Immunofluorescent analysis of Transfected HEK-293 cells using Catalog No:117334(MYC-tag Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The antibody recognizes the MYC tag EQKLISEEDL.
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References
- Xu D, Zhang T, Xiao J. Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon. Autophagy. 11(4):617-28. 2015.
- Xu D, Shan B, Lee BH. Phosphorylation and activation of ubiquitin-specific protease-14 by Akt regulates the ubiquitin-proteasome system. eLife. 4:. 2015.
- Ding X, Jiang W, Zhou P. Mixed Lineage Leukemia 5 (MLL5) Protein Stability Is Cooperatively Regulated by O-GlcNac Transferase (OGT) and Ubiquitin Specific Protease 7 (USP7). PloS one. 10(12):e0145023. 2015.
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