-
Product Name
MYC tag antibody
- Documents
-
Description
MYC tag Mouse Monoclonal antibody. Positive IP detected in Transfected HEK-293 cells. Positive WB detected in recombinant proein. Positive IF detected in Transfected HEK-293 cells.
-
Tested applications
ELISA, WB, IP, IF
-
Species reactivity
Human,Mouse; other species not tested.
-
Isotype
Mouse IgG1
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: 3XMYC tag). Purification method: Protein A purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:2000-1:20000
IP: 1:5000-1:50000
IF: 1:10-1:100
-
Validations
Western blot of Myc-tagged fusion protein with anti-Myc-tag (Catalog No:117332) at various dilutions.
IP Result of anti-MYC-tag (IP:Catalog No:117332, 4ug; Detection:Catalog No:117332 1:10000) with Transfected HEK-293 cells lysate 300ug.
Immunofluorescent analysis of Transfected HEK-293 cells using Catalog No:117332(MYC-tag Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
-
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
-
References
- Liu Z, Luo X, Liu L. Histone acetyltransferase p300 promotes MKL1-mediated transactivation of catechol-O-methyltransferase gene. Acta biochimica et biophysica Sinica. 45(12):1002-10. 2013.
- Wortham NC, Martinez M, Gordiyenko Y, Robinson CV, Proud CG. Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 28(5):2225-37. 2014.
- Chen XQ, Zhu JX, Wang Y, Zhang X, Bao L. CaMKIIα and caveolin-1 cooperate to drive ATP-induced membrane delivery of the P2X3 receptor. Journal of molecular cell biology. 6(2):140-53. 2014.
- Fu Y, Lv P, Yan G. MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells. Scientific reports. 5:17186. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"