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Product Name
MXI1 Polyclonal Antibody
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Description
Polyclonal antibody to MXI1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MXI1 antibody; MAD2 antibody; MXD2 antibody; MXI antibody; bHLHc11 antibody; max-interacting protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 76-295 of human MXI1 (NP_569157.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - MXI1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MXI1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - MXI1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using MXI1 antibody . Blue: DAPI for nuclear staining.
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Background
Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"