MXI1 Polyclonal Antibody

Cat.#: 164973

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Product Information

  • Product Name
    MXI1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to MXI1
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    MXI1 antibody; MAD2 antibody; MXD2 antibody; MXI antibody; bHLHc11 antibody; max-interacting protein 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 76-295 of human MXI1 (NP_569157.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:100
  • Validations

    Western blot - MXI1 Polyclonal Antibody

    Western blot - MXI1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using MXI1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunofluorescence - MXI1 Polyclonal Antibody

    Immunofluorescence - MXI1 Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using MXI1 antibody . Blue: DAPI for nuclear staining.

  • Background
    Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"