MUS81 Polyclonal Antibody

Cat.#: 165055

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Product Information

  • Product Name
    MUS81 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to MUS81
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    MUS81 antibody; SLX3 antibody; crossover junction endonuclease MUS81 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MUS81 (NP_079404.3).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:100
  • Validations

    Western blot - MUS81 Polyclonal Antibody

    Western blot - MUS81 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using MUS81 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunohistochemistry - MUS81 Polyclonal Antibody

    Immunohistochemistry - MUS81 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat brain using MUS81 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - MUS81 Polyclonal Antibody

    Immunohistochemistry - MUS81 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse brain using MUS81 antibody at dilution of 1:100 (40x lens).

  • Background
    Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"