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Product Name
MUS81 Polyclonal Antibody
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Description
Polyclonal antibody to MUS81
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MUS81 antibody; SLX3 antibody; crossover junction endonuclease MUS81 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MUS81 (NP_079404.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - MUS81 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MUS81 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - MUS81 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using MUS81 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MUS81 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using MUS81 antibody at dilution of 1:100 (40x lens).
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Background
Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"