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Product Name
MTHFD2 antibody
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Description
MTHFD2 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in Jurkat cells, mouse testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 38 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MTHFD2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MTHFD2 recombinant protein (Accession Number: NM_006636). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112778(MTHFD2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112778(MTHFD2 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-MTHFD2 (IP:Catalog No:112778, 4ug; Detection:Catalog No:112778 1:300) with mouse testis tissue lysate 4000ug.
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Background
MTHFD2 represents methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
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References
- Sugiyama K, Ebinuma H, Nakamoto N. Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus. PloS one. 9(4):e94460. 2014.
- Mitsuishi Y, Taguchi K, Kawatani Y. Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. Cancer cell. 22(1):66-79. 2012.
- Piskounova E, Agathocleous M, Murphy MM. Oxidative stress inhibits distant metastasis by human melanoma cells. Nature. 527(7577):186-91. 2015.
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