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Product Name
MTCO2 Polyclonal Antibody
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Description
Polyclonal antibody to MTCO2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MT-CO2 antibody; COII antibody; MTCO2 antibody; COX2 antibody; mitochondrially encoded cytochrome c oxidase II antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse MTCO2 (NP_904331.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - MTCO2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MTCO2 antibody at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunofluorescence - MTCO2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using MTCO2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"