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Product Name
MTCO2 antibody
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Description
MTCO2 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HepG2 cells, HeLa cells, human placenta tissue, mouse brain tissue, mouse liver tissue, rat liver tissue. Positive IHC detected in human colon cancer tissue, human breast cancer tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 23-26 kDa
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Tested applications
ELISA, IHC, WB, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
COII antibody; COX2 antibody; COXII antibody; cytOChrome c oxidase II antibody; CytOChrome c oxidase subunit 2 antibody; MT CO2 antibody; MTCO2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: YP_003024029). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112767(COX2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112767(COX2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112767(COX2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-COX2 (IP:Catalog No:112767, 4ug; Detection:Catalog No:112767 1:1000) with HeLa cells lysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug COX2 antibody (Catalog No:112767, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
COX2, also named as COII, COXII and MTCO2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to COX2.
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References
- Towheed A, Markantone DM, Crain AT, Celotto AM, Palladino MJ. Small mitochondrial-targeted RNAs modulate endogenous mitochondrial protein expression in vivo. Neurobiology of disease. 69:15-22. 2014.
- Mohar I, Stamper BD, Rademacher PM, White CC, Nelson SD, Kavanagh TJ. Acetaminophen-induced liver damage in mice is associated with gender-specific adduction of peroxiredoxin-6. Redox biology. 2:377-87. 2014.
- Zhang J, Wang C, Chen X. EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer. The EMBO journal. 34(23):2953-70. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"