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Product Name
MTAP antibody
- Documents
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Description
MTAP Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in MCF7 cells, human heart tissue, NIH/3T3 cells, Raji cells. Positive IP detected in MCF-7 cells. Observed molecular weight by Western-blot: 31 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human, Rat, Mouse; other species not tested.
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Alternative names
c86fus antibody; MSAP antibody; MTA phosphorylase antibody; MTAP antibody; MTAPase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MTAP recombinant protein (Accession Number: BC018625). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112765(MTAP antibody) at dilution of 1:50 (under 10x lens)
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:112765(MTAP antibody) at dilution of 1:500
IP Result of anti-MTAP (IP:Catalog No:112765, 3ug; Detection:Catalog No:112765 1:500) with MCF-7 cells lysate 3250ug.
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Background
MTAP represents methylthioadenosine phosphorylase
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References
- Huang HY, Li SH, Yu SC. Homozygous deletion of MTAP gene as a poor prognosticator in gastrointestinal stromal tumors. Clinical cancer research : an official journal of the American Association for Cancer Research. 15(22):6963-72. 2009.
- Sommer J, Itani DM, Homlar KC. Methylthioadenosine phosphorylase and activated insulin-like growth factor-1 receptor/insulin receptor: potential therapeutic targets in chordoma. The Journal of pathology. 220(5):608-17. 2010.
- Kim J, Kim MA, Min SY, Jee CD, Lee HE, Kim WH. Downregulation of methylthioadenosin phosphorylase by homozygous deletion in gastric carcinoma. Genes, chromosomes & cancer. 50(6):421-33. 2011.
- Zimling ZG, Jørgensen A, Santoni-Rugiu E. The diagnostic value of immunohistochemically detected methylthioadenosine phosphorylase deficiency in malignant pleural mesotheliomas. Histopathology. 60(6B):E96-105. 2012.
- Chidlow G, Wood JP, Sharma S. Ocular expression and distribution of products of the POAG-associated chromosome 9p21 gene region. PloS one. 8(9):e75067. 2013.
- Sepiashvili L, Waggott D, Hui A. Integrated omic analysis of oropharyngeal carcinomas reveals human papillomavirus (HPV)-dependent regulation of the activator protein 1 (AP-1) pathway. Molecular & cellular proteomics : MCP. 13(12):3572-84. 2014.
- Gambichler T, Scola N, Bechara FG. Significantly decreased methylthioadenosine phosphorylase expression in malignant melanoma. The American Journal of dermatopathology. 34(7):777-9. 2012.
- Li CF, Fang FM, Kung HJ. Downregulated MTAP expression in myxofibrosarcoma: A characterization of inactivating mechanisms, tumor suppressive function, and therapeutic relevance. Oncotarget. 5(22):11428-41. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"