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Product Name
MTA3 antibody
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Description
MTA3 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, Jurkat cells, MCF7 cells, mouse brain tissue, mouse thymus tissue, Raji cells. Positive IP detected in Raji cells. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 62 kDa,68 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA1266 antibody; MTA3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MTA3 recombinant protein (Accession Number: NM_020744). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112764(MTA3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:112764(MTA3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MTA3 antibody Catalog No:112764 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MTA3 (IP:Catalog No:112764, 4ug; Detection:Catalog No:112764 1:500) with Raji cells lysate 2800ug.
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Background
Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.
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References
- Li H, Sun L, Xu Y. Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer. PloS one. 8(6):e66679. 2013.
- Chu H, Chen X, Wang H. MiR-495 regulates proliferation and migration in NSCLC by targeting MTA3. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 35(4):3487-94. 2014.
- Qin J, Whyte WA, Anderssen E. The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development. Cell stem cell. 11(3):319-32. 2012.
- Shan S, Hui G, Hou F. Expression of metastasis-associated protein 3 in human brain glioma related to tumor prognosis. Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology. 36(10):1799-804. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"