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Product Name
MT-ND5 Polyclonal Antibody
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Description
Polyclonal antibody to MT-ND5
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
MT-ND5 antibody; MTND5 antibody; ND5 antibody; NADH dehydrogenase, subunit 5 (complex I) antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of mouse MT-ND5 (NP_904338.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - MT-ND5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MT-ND5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - MT-ND5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using MT-ND5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MT-ND5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using MT-ND5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MT-ND5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using MT-ND5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MT-ND5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate cancer using MT-ND5 antibody at dilution of 1:100 (40x lens).
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Background
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"