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MT-ATP8 Polyclonal Antibody

Cat.#: 163554

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Product Information

  • Product Name
    MT-ATP8 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to MT-ATP8
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ATPase8 antibody; MTATP8 antibody; ATP8 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant protein of human MT-ATP8.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:100
  • Validations

    Western blot - MT-ATP8 Polyclonal Antibody

    Western blot - MT-ATP8 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using MT-ATP8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 2min.

    Immunofluorescence - MT-ATP8 Polyclonal Antibody

    Immunofluorescence - MT-ATP8 Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using MT-ATP8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - MT-ATP8 Polyclonal Antibody

    Immunofluorescence - MT-ATP8 Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using MT-ATP8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane (By similarity).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"