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Product Name
MSLN Polyclonal Antibody
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Description
Polyclonal antibody to MSLN
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
MSLN antibody; MPF antibody; SMRP antibody; mesothelin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-296 of human MSLN (NP_001170826.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - MSLN Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MSLN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - MSLN Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using MSLN antibody . Blue: DAPI for nuclear staining.
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Background
Membrane-anchored forms may play a role in cellular adhesion.; Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"