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Product Name
MSH2 antibody
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Description
MSH2 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A2780 cells. Positive FC detected in HeLa cells. Positive IF detected in Hela cells. Positive IHC detected in human colon cancer tissue, human tonsil tissue. Observed molecular weight by Western-blot: 100 kDa-105 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
COCA1 antibody; FCC1 antibody; hMSH2 antibody; HNPCC antibody; HNPCC1 antibody; LCFS2 antibody; MSH2 antibody; MutS protein homolog 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MSH2 recombinant protein (Accession Number: NM_000251). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112867(MSH2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112867(MSH2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112867(MSH2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using MSH2 antibody Catalog No:112867 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MSH2 (IP:Catalog No:112867, 5ug; Detection:Catalog No:112867 1:500) with HeLa cells lysate 2000ug.
1X10^6 HeLa cells were stained with 0.2ug MSH2 antibody (Catalog No:112867, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.
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References
- Jin P, Lu XJ, Sheng JQ. Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells. Cancer prevention research (Philadelphia, Pa.). 3(8):910-6. 2010.
- Mo C, Dai Y, Kang N, Cui L, He W. Ectopic expression of human MutS homologue 2 on renal carcinoma cells is induced by oxidative stress with interleukin-18 promotion via p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways. The Journal of biological chemistry. 287(23):19242-54. 2012.
- He YQ, Sheng JQ, Ling XL. Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells. Experimental & molecular medicine. 44(12):723-32. 2012.
- Yamagishi H, Imai Y, Okamura T. Aberrant cytokeratin expression as a possible prognostic predictor in poorly differentiated colorectal carcinoma. Journal of gastroenterology and hepatology. 28(12):1815-22. 2013.
- Nguyen TT, Hachisuga T, Urabe R. Significance of p53 expression in background endometrium in endometrial carcinoma. Virchows Archiv : an international journal of pathology. 466(6):695-702. 2015.
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