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Product Name
MSH2 antibody
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Description
MSH2 Mouse Monoclonal antibody. Positive WB detected in Colo320 cells, A2780 cells, HeLa cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 100-105 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
COCA1 antibody; FCC1 antibody; hMSH2 antibody; HNPCC antibody; HNPCC1 antibody; LCFS2 antibody; MSH2 antibody; MutS protein homolog 2 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of MSH2 recombinant protein (Accession Number: NM_000251). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:107430(MSH2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:107430(MSH2 Antibody) at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:107430(MSH2 Antibody) at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"