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Product Name
MRPS30 Polyclonal Antibody
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Description
Polyclonal antibody to MRPS30
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
MRPS30 antibody; MRP-S30 antibody; PAP antibody; PDCD9 antibody; S30mt antibody; mitochondrial ribosomal protein S30 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-439 of human MRPS30 (NP_057724.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200 -
Validations
Western blot - MRPS30 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MRPS30 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - MRPS30 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using MRPS30 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MRPS30 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using MRPS30 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - MRPS30 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using MRPS30 antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - MRPS30 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug MRPS30 antibody . Western blot was performed from the immunoprecipitate using MRPS30 antibody at a dilition of 1:1000.
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