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Product Name
MRPS15 antibody
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Description
MRPS15 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human spleen tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human testis tissue. Observed molecular weight by Western-blot: 30kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DC37 antibody; FLJ11564 antibody; MPR S15 antibody; MRP S15 antibody; MRPS15 antibody; RPMS15 antibody; S15mt antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MRPS15 recombinant protein (Accession Number: NM_031280). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112842(MRPS15 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human spleen using Catalog No:112842(MRPS15 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:112842(MRPS15 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MRPS15 antibody Catalog No:112842 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
MRPS15, also named as RPMS15 or DC37, is a 257 amino acid protein, which belongs to the ribosomal protein S15P family. MRPS15 localizes in the Mitochondrion and is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. MRPS15 is involved into the mitochondrial proteins biosynthesis and ribosomal biogenesis.
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References
- Lee KW, Okot-Kotber C, LaComb JF, Bogenhagen DF. Mitochondrial ribosomal RNA (rRNA) methyltransferase family members are positioned to modify nascent rRNA in foci near the mitochondrial DNA nucleoid. The Journal of biological chemistry. 288(43):31386-99. 2013.
- Bogenhagen DF, Martin DW, Koller A. Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids. Cell metabolism. 19(4):618-29. 2014.
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