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Product Name
MRPP3 antibody
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Description
MRPP3 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, L02 cells, mouse liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 65-67 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0391 antibody; MRPP3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MRPP3 recombinant protein (Accession Number: XM_017021835). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112838(KIAA0391 antibody) at dilution of 1:800
Immunofluorescent analysis of HepG2 cells, using KIAA0391 antibody Catalog No:112838 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694)
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References
- Bogenhagen DF, Martin DW, Koller A. Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids. Cell metabolism. 19(4):618-29. 2014.
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