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Product Name
MRPL53 antibody
- Documents
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Description
MRPL53 Rabbit Polyclonal antibody. Positive IF detected in A549 cells. Positive IHC detected in human skin cancer tissue. Positive WB detected in A549 cells. Observed molecular weight by Western-blot: 12 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ90728 antibody; L53mt antibody; MRP L53 antibody; MRPL53 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MRPL53 recombinant protein (Accession Number: NM_053050). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:112835(MRPL53 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using Catalog No:112835(MRPL53 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using Catalog No:112835(MRPL53 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed A549 cells using Catalog No:112835(MRPL53 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MRPL53 represents mitochondrial ribosomal protein L53
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"