MRPL45 antibody

Cat.#: 112829

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Product Information

  • Product Name
    MRPL45 antibody
  • Documents
  • Description
    MRPL45 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, human brain tissue, human liver tissue, mouse liver tissue. Positive IF detected in HeLa cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 35kd
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    L45mt antibody; MRPL45 antibody; MRP-L45 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MRPL45 recombinant protein (Accession Number: BC006235). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112829(MRPL45 antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112829(MRPL45 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112829(MRPL45 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112829(MRPL45 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112829(MRPL45 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112829(MRPL45 antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-MRPL45 (IP:Catalog No:112829, 3ug; Detection:Catalog No:112829 1:500) with HeLa cells lysate 2000ug.

    IP Result of anti-MRPL45 (IP:Catalog No:112829, 3ug; Detection:Catalog No:112829 1:500) with HeLa cells lysate 2000ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:112829(MRPL45 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:112829(MRPL45 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    MRPL45 represents mitochondrial ribosomal protein L45
  • References
    • Dalla Rosa I, Durigon R, Pearce SF. MPV17L2 is required for ribosome assembly in mitochondria. Nucleic acids research. 42(13):8500-15. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"