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Product Name
MRPL12 antibody
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Description
MRPL12 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, COLO 320 cells, MCF7 cells, mouse skeletal muscle tissue. Positive IP detected in HeLa cells. Positive IF detected in Hela cells. Positive IHC detected in human colon cancer tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 21kd
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
5c5 2 antibody; FLJ60124 antibody; L12mt antibody; MRP L12 antibody; MRP L31/34 antibody; MRPL12 antibody; MRPL7 antibody; MRPL7/L12 antibody; RPML12 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MRPL12 recombinant protein (Accession Number: NM_002949). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112811(MRPL12 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112811(MRPL12 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using MRPL12 antibody Catalog No:112811 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-MRPL12 (IP:Catalog No:112811, 4ug; Detection:Catalog No:112811 1:500) with HeLa cells lysate 2000ug.
1X10^6 HeLa cells were stained with 0.2ug MRPL12 antibody (Catalog No:112811, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.
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References
- Dalla Rosa I, Durigon R, Pearce SF. MPV17L2 is required for ribosome assembly in mitochondria. Nucleic acids research. 42(13):8500-15. 2014.
- Nouws J, Goswami AV, Bestwick M, McCann BJ, Surovtseva YV, Shadel GS. Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import. The Journal of biological chemistry. 291(2):989-97. 2016.
- Surovtseva YV, Shadel GS. Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis. Nucleic acids research. 41(4):2479-88. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"