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Product Name
MPZL2 antibody
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Description
MPZL2 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 24kd and 32kd
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Epithelial V like antigen 1 antibody; EVA antibody; EVA1 antibody; MPZL2 antibody; myelin protein zero like 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MPZL2 recombinant protein (Accession Number: NM_144765). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112761(MPZL2 antibody) at dilution of 1:800
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Background
MPZL2 (myelin protein zero-like 2), also named as EVA (epithelial V-like antigen) or EVA1, is a type 1 transmembrane protein of the myelin P0 protein family, containing an Ig-like V-type (immunoglobulin-like) domain and two potential glycosylation sites. MPZL2 is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression. Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis. The gene of MPZL2 maps to chromosome 11q24, and encodes a 215-amino acid protein with a predicted unmodified molecular weight of 24.5 kDa. This antibody can be used to detect endogenous MPZL2, however, the reason for the additional band of 32 kDa is still unknown.
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References
- Wojcik E, Carrithers LM, Carrithers MD. Characterization of epithelial V-like antigen in human choroid plexus epithelial cells: potential role in CNS immune surveillance. Neuroscience letters. 495(2):115-20. 2011.
- Wu J, Boström P, Sparks LM. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell. 150(2):366-76. 2012.
- Timms RT, Duncan LM, Tchasovnikarova IA. Haploid genetic screens identify an essential role for PLP2 in the downregulation of novel plasma membrane targets by viral E3 ubiquitin ligases. PLoS pathogens. 9(11):e1003772. 2013.
- Wright E, Rahgozar K, Hallworth N, Lanker S, Carrithers MD. Epithelial V-like antigen mediates efficacy of anti-alpha₄ integrin treatment in a mouse model of multiple sclerosis. PloS one. 8(8):e70954. 2013.
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