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MPZ,P0 antibody

Cat.#: 112759

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Product Information

  • Product Name
    MPZ,P0 antibody
  • Documents
  • Description
    MPZ,P0 Rabbit Polyclonal antibody. Positive IF detected in myelinating SCs cells. Positive WB detected in Schwann cells. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CHM antibody; CMT1 antibody; CMT1B antibody; CMT2I antibody; CMT2J antibody; CMT4E antibody; CMTDI3 antibody; DSS antibody; HMSNIB antibody; MPP antibody; MPZ antibody; Myelin peripheral protein antibody; Myelin protein P0 antibody; myelin protein zero antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MPZ,P0 recombinant protein (Accession Number: BC006491). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    WB result from Chattopadhyay S (PMID:19229995); Schwann cell differentiation was induced with dbcAMP (500 μM) for 48 h, followed by treatment with rhMMP-9 (100 nM) for 15 min. A, rhMMP-9 stimulates transient ERK1/2 activation over a 1 h period. Successful Schwann cell differentiation was confirmed by myelin protein zero (P0) expression, β-actin was used as loading control.

    WB result from Chattopadhyay S (PMID:19229995); Schwann cell differentiation was induced with dbcAMP (500 μM) for 48 h, followed by treatment with rhMMP-9 (100 nM) for 15 min. A, rhMMP-9 stimulates transient ERK1/2 activation over a 1 h period. Successful Schwann cell differentiation was confirmed by myelin protein zero (P0) expression, β-actin was used as loading control.

    IF result from Huaqing Liu (PMID:20448483);Dual immunofluorescence for BrdU (red) with phenotypic cell markers (green) in distal segments of MMPi-treated promotes Schwann cell (SC) mitosis in regenerating nerves. myelinating SCs (mSCs) (P0)

    IF result from Huaqing Liu (PMID:20448483);Dual immunofluorescence for BrdU (red) with phenotypic cell markers (green) in distal segments of MMPi-treated promotes Schwann cell (SC) mitosis in regenerating nerves. myelinating SCs (mSCs) (P0)

  • Background
    MPZ (myelin protein zero), also known as P0, a transmembrane glycoprotein (~30 kDa), is a member of the immunoglobulin supergene family. Synthesized by myelin-forming Schwann cells, MPZ is the major structural protein component of myelin in the peripheral nervous system. It is involved in formation and maintenance of compact myelin, and plays a role in the creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. More than 120 mutations detected in the gene of MPZ cause various forms of hereditary neuropathy, which include Charcot-Marie-Tooth disease type 1B (CMT1B), CMT2, Dejerine-Sottas syndrome (DSS), and congenital hypomyelination neuropathy (CHN). This antibody can recognize endogenous MPZ, and can be used as a marker of myelinating Schwann cells.
  • References
    • Kobayashi H, Chattopadhyay S, Kato K. MMPs initiate Schwann cell-mediated MBP degradation and mechanical nociception after nerve damage. Molecular and cellular neurosciences. 39(4):619-27. 2008.
    • Liu H, Kim Y, Chattopadhyay S, Shubayev I, Dolkas J, Shubayev VI. Matrix metalloproteinase inhibition enhances the rate of nerve regeneration in vivo by promoting dedifferentiation and mitosis of supporting schwann cells. Journal of neuropathology and experimental neurology. 69(4):386-95. 2010.
    • Chattopadhyay S, Shubayev VI. MMP-9 controls Schwann cell proliferation and phenotypic remodeling via IGF-1 and ErbB receptor-mediated activation of MEK/ERK pathway. Glia. 57(12):1316-25. 2009.
    • Schwab AJ, Ebert AD. Sensory neurons do not induce motor neuron loss in a human stem cell model of spinal muscular atrophy. PloS one. 9(7):e103112. 2014.
    • Eckharter C, Junker N, Winter L. Schwann Cell Expressed Nogo-B Modulates Axonal Branching of Adult Sensory Neurons Through the Nogo-B Receptor NgBR. Frontiers in cellular neuroscience. 9:454. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"