Mouse Ephrin-A1/EFNA1 (Fc Tag) recombinant protein

Mouse Ephrin-A1/EFNA1 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

EPH-related receptor tyrosine kinase ligand 1 (abbreviated as Ephrin-A1) also known as ligand of eph-related kinase 1 or EFNA1, is a member of the ephrin (EPH) family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A1/EFNA1 and its Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A1 and one of its receptor EphA2 were expressed in xenograft endothelial cells and also tumor cells and play a role in human cancers, at least in part by influencing tumor neovascularization.

CD99 antigen-like protein 2

Q8BIF0

Cd99l2; Mic2l1

Human Cells

A DNA sequence encoding the mouse EFNA1 (NP_612182.1) without the pro peptide (Met 1-Ser 182) was fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The secreted recombinant mouse EFNA1/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 405 amino acids and has a predicted molecular mass of 46.4 kDa. As a result of glycosylation, the apparent molecular mass of rm EFNA1/Fc monomer is approximately 53 kDa in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA2 at 2 μg/ml (100 μl/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/ml.