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Product Name
Mouse anti HA-Tag mAb
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Description
Monoclonal antibody to Mouse anti HA-Tag
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Tested applications
WB, IF, IP
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Isotype
Mouse IgG
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Preparation
Antigen: A synthetic peptide of YPYDVPDYA (HA-tag)-KLH
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:2000 - 1:5000
IF 1:50 - 1:500
IP 1:20 - 1:50 -
Validations
Western blot - Mouse anti HA-Tag mAb
Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody at different dilution. Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - Mouse anti HA-Tag mAb
Immunofluorescence analysis of 293T cells using Mouse anti HA-Tag mAb . Blue: DAPI for nuclear staining.
Immunoprecipitation - Mouse anti HA-Tag mAb
Immunoprecipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody . A mock served as negative control and over-expressed 293T cell lysate served as positive control.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"