Mouse anti HA-Tag mAb

Cat.#: 166295

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Product Information

  • Product Name
    Mouse anti HA-Tag mAb
  • Documents
  • Description
    Monoclonal antibody to Mouse anti HA-Tag
  • Tested applications
    WB, IF, IP
  • Alternative names
    antibody
  • Isotype
    Mouse IgG
  • Preparation
    Antigen: A synthetic peptide of YPYDVPDYA (HA-tag)-KLH
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:2000 - 1:5000
    IF 1:50 - 1:500
    IP 1:20 - 1:50
  • Validations

    Western blot - Mouse anti HA-Tag mAb

    Western blot - Mouse anti HA-Tag mAb

    Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody at different dilution. Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

    Immunofluorescence - Mouse anti HA-Tag mAb

    Immunofluorescence - Mouse anti HA-Tag mAb

    Immunofluorescence analysis of 293T cells using Mouse anti HA-Tag mAb . Blue: DAPI for nuclear staining.

    Immunoprecipitation - Mouse anti HA-Tag mAb

    Immunoprecipitation - Mouse anti HA-Tag mAb

    Immunoprecipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody . A mock served as negative control and over-expressed 293T cell lysate served as positive control.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"