Mouse ALK-3 / BMPR1A (His & Fc Tag) recombinant protein

Mouse ALK-3 / BMPR1A (His & Fc Tag) recombinant protein

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Activin receptor-Like Kinase 3 (ALK-3), also known as Bone Morphogenetic Protein Receptor, type IA (BMPR1A), is a type I receptor for bone morphogenetic proteins (BMPs) which belong to the transforming growth factor beta (TGF-β) superfamily. The BMP receptors form a subfamily of transmembrane serine/threonine kinases including the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. ALK-3/BMPR1A is expressed in the epithelium during branching morphogenesis. Deletion of BMPR1A in the epithelium with an Sftpc-cre transgene leads to dramatic defects in lung development. ALK-3 and ALK-6 share a high degree of homology, yet possess distinct signaling roles. The transforming growth factor (TGF)-beta type III receptor (TbetaRIII) enhanced both ALK-3 and ALK-6 signaling. TbetaRIII associated with ALK-3 primarily through their extracellular domains, whereas its interaction with ALK-6 required both the extracellular and cytoplasmic domains. ALK-3 plays an essential role in the formation of embryonic ventral abdominal wall, and abrogation of BMP signaling activity due to gene mutations in its signaling components could be one of the underlying causes of omphalocele at birth. The type IA BMP receptor, ALK-3 was specifically required at mid-gestation for normal development of the trabeculae, compact myocardium, interventricular septum, and endocardial cushion. Cardiac muscle lacking ALK-3 was specifically deficient in expressing TGFbeta2, an established paracrine mediator of cushion morphogenesis. Hence, ALK-3 is essential, beyond just the egg cylinder stage, for myocyte-dependent functions and signals in cardiac organogenesis.

Bone morphogenetic protein receptor type-1A

BMPR; ACVRLK3; 10Q23DEL; SKR5; ALK3; BMPR1A; BMPR-IA; CD292; ALK-3; 1110037I22RIK; AU045487

Q53Z43

Bmpr1a; Acvrlk3; Bmpr

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Arg 152) of mouse ALK3 (NP_033888.2) precursor was fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse ALK3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 376 amino acids and has a predicted molecular mass of 42 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ALK3/Fc monomer is approximately 60 kDa due to glycosylation.

> 95 % as determined by SDS-PAGE

Measured by its ability to inhibit BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED₅₀ for this effect is typically 0.1-0.3 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.