Mouse Smad3 (His & GST Tag) recombinant protein

Mouse Smad3 (His & GST Tag) recombinant protein

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Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).

Mothers against decapentaplegic homolog 3

HSMAD3; RP23-63E8.1; HSPC193; HMAD-3; SMAD; SMAD 3; MAD3; LDS1C; MAD PROTEIN HOMOLOG; MAD HOMOLOG JV15-2; MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 3; MOTHERS AGAINST DPP HOMOLOG 3; MADH3; LDS3; AU022421; JV15-2; SMAD FAMILY MEMBER 3; HST17436; SMAD3; SMA- AND MAD-RELATED PROTEIN 3; MAD HOMOLOG 3; MAD; MMAD3

P84025

Smad3; Madh3

Baculovirus-Insect Cells

A DNA sequence encoding the mouse SMAD3 (P84025) (Met1-Ser425) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Mouse

The recombinant mouse SMAD3/GST chimera consists of 662 amino acids and has a calculated molecular mass of 75.9 kDa. The recombinant protein migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE