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Product Name
MNAT1 Polyclonal Antibody
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Description
Polyclonal antibody to MNAT1
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Tested applications
WB, IP
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Species reactivity
Human, Mouse
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Alternative names
MNAT1 antibody; CAP35 antibody; MAT1 antibody; RNF66 antibody; TFB3 antibody; CDK-activating kinase assembly factor MAT1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MNAT1 (NP_002422.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:50 - 1:200 -
Validations
Western blot - MNAT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MNAT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation - MNAT1 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug MNAT1 antibody . Western blot was performed from the immunoprecipitate using MNAT1 antibody at a dilition of 1:1000.
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Background
Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"