Product Name

MMP3 antibody

Documents

Description

MMP3 Rabbit Polyclonal antibody. Positive WB detected in A549 cells, A2780 cells, HeLa cells, human skeletal muscle tissue. Positive IHC detected in human breast cancer tissue, human stomach cancer tissue. Positive IF detected in A549 cells. Observed molecular weight by Western-blot: 45-47 kDa

Tested applications

ELISA, WB, IHC, IF

Species reactivity

Human, Mouse, Rat; other species not tested.

Alternative names

CHDS6 antibody; Matrix metalloproteinase 3 antibody; MMP 3 antibody; MMP3 antibody; SL 1 antibody; STMY antibody; STMY1 antibody; STR1 antibody; Stromelysin 1 antibody; Transin 1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of MMP3 recombinant protein (Accession Number: NM_002422). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:112706(MMP3 antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112706(MMP3 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112706(MMP3 antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of A549 cells using Catalog No:112706(MMP3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

References

Fong G, Backman LJ, Hart DA, Danielson P, McCormack B, Scott A. Substance P enhances collagen remodeling and MMP-3 expression by human tenocytes. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 31(1):91-8. 2013.
Ding Q, Zhong H, Qi Y. Anti-arthritic effects of crocin in interleukin-1β-treated articular chondrocytes and cartilage in a rabbit osteoarthritic model. Inflammation research : official journal of the European Histamine Research Society ...
Zhang M, Liu Y, Feng H. CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2. The American journal of pathology. 182(2):565-76. 2013.
Yoshioka Y, Kozawa E, Urakawa H. Suppression of hyaluronan synthesis alleviates inflammatory responses in murine arthritis and in human rheumatoid synovial fibroblasts. Arthritis and rheumatism. 65(5):1160-70. 2013.
Ding QH, Cheng Y, Chen WP, Zhong HM, Wang XH. Celastrol, an inhibitor of heat shock protein 90β potently suppresses the expression of matrix metalloproteinases, inducible nitric oxide synthase and cyclooxygenase-2 in primary human osteoarthritic chondrocytes. European journal of pharmacology. 708(1-3):1-7. 2013.
Zuo X, Pan W, Feng T, Shi X, Dai J. Matrix metalloproteinase 3 promotes cellular anti-dengue virus response via interaction with transcription factor NFκB in cell nucleus. PloS one. 9(1):e84748. 2014.
Mehner C, Miller E, Khauv D. Tumor cell-derived MMP3 orchestrates Rac1b and tissue alterations that promote pancreatic adenocarcinoma. Molecular cancer research : MCR. 12(10):1430-9. 2014.
Ma F, Li H, Wang H. Enriched CD44(+)/CD24(-) population drives the aggressive phenotypes presented in triple-negative breast cancer (TNBC). Cancer letters. 353(2):153-9. 2014.

MMP3 antibody

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