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Product Name
MMP1 Polyclonal Antibody
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Description
Polyclonal antibody to MMP1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
MMP1 antibody; CLG antibody; CLGN antibody; interstitial collagenase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human MMP1 (NP_002412.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - MMP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MMP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - MMP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human cervical carcinoma using MMP1 antibody at dilution of 1:300 (40x lens).
Immunohistochemistry - MMP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using MMP1 antibody at dilution of 1:200 (40x lens).
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Background
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"