-
Product Name
MLPH Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to MLPH
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
MLPH antibody; SLAC2-A antibody; melanophilin antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 301-600 of human MLPH (NP_077006.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - MLPH Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MLPH antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - MLPH Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using MLPH antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - MLPH Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using MLPH antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"