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Product Name
MKL1 antibody
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Description
MKL1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human tonsillitis tissue, human liver cancer tissue, human small intestine tissue. Positive WB detected in HepG2 cells, HeLa cells. Observed molecular weight by Western-blot: 145 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
BSAC antibody; KIAA1438 antibody; MAL antibody; MKL/myOCardin like protein 1 antibody; MKL1 antibody; MRTF A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MKL1 recombinant protein (Accession Number: BC115039). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112666(MKL1 antibody) at dilution of 1:3000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:112666(MKL1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:112666(MKL1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:112666(MKL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MKL1 represents megakaryoblastic leukemia (translocation) 1
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References
- Shu XZ, Zhang LN, Zhang R. Histone acetyltransferase p300 promotes MRTF-A-mediates transactivation of VE-cadherin gene in human umbilical vein endothelial cells. Gene. 563(1):17-23. 2015.
- He H, Wang D, Yao H. Transcriptional factors p300 and MRTF-A synergistically enhance the expression of migration-related genes in MCF-7 breast cancer cells. Biochemical and biophysical research communications. 467(4):813-20. 2015.
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