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Product Name
MIXL1 Polyclonal Antibody
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Description
Polyclonal antibody to MIXL1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
MIXL1 antibody; MILD1 antibody; MIX antibody; MIXL antibody; homeobox protein MIXL1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human MIXL1.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - MIXL1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines,using MIXL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5min.
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Background
Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"