Human MAP4K2/GC Kinase (His & GST Tag) recombinant protein
Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion.
Mitogen-activated protein kinase kinase kinase kinase 2
Protein short names
MGC143894; GCK; BL44; RAB8IP; AI385662; MAP4K2; MGC143893; GC KINASE
MAP4K2; GCK; RAB8IP
A DNA sequence encoding the human MAP4K2 (AAH47865.1) (Met 1-Tyr 812) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
The recombinant human MAP4K2/GST chimera consists of 1049 amino acids and has a calculated molecular mass of 119 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.
> 87 % as determined by SDS-PAGE
Kinase activity untested
Human MAP4K2 / GC Kinase Protein (His & GST Tag) SDS-PAGE
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