Human MAPKAPK3 (GST Tag) recombinant protein

Human MAPKAPK3 (GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The MAPKAP kinases are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. Mitogen-activated protein (MAP) kinase-activated protein kinase 3, also known as MAPKAPK-3 and 3pK, is a member of the Ser/Thr protein kinase family. It is Widely expressed in human tissues, with a higher expression level observed in heart and skeletal muscle. No expression in brain. MAPKAPK-3 is unique since it was shown to be activated by three members of the MAPK family, namely extracellular-signal-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK). It is highly activated both by mitogens and by stress-inducing agents or proinflammatory cytokines, and translocates to the cytoplasm from nucleus. MAPKAPK-3 is exclusively activated via the classical MAPK cascade, while stress-induced activation of MAPKAPK-3 is mainly mediated by p38, however the mechanism defining the specificity remains unknown.

Mitogen-activated protein kinase-activated protein kinase 3, isoform CRA_a

MK-3; MAPKAP3; MAPKAPK3; MK3; MAPKAP-K3; AI874665; MAPKKAPK3; MAPKAPK-3; 3PK

Q16644

MAPKAPK3; hCG_17458

Baculovirus-Insect Cells

A DNA sequence encoding the full length of human MAPKAPK3 (NP_004626.1) (Met 1-Gln 382) was expressed with the GST tag at the N-terminus.

Human

The recombinant human MAPKAPK3/GST chimera consists of 607 amino acids and predicts a molecular mass of 69 kDa which is also estimated by SDS-PAGE.

> 90 % as determined by SDS-PAGE

Kinase activity untested