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Product Name
MITD1 antibody
- Documents
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Description
MITD1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, NIH/3T3 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MITD1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MITD1 recombinant protein (Accession Number: NM_138798). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112660(MITD1 antibody) at dilution of 1:500
IP Result of anti-MITD1 (IP:Catalog No:112660, 4ug; Detection:Catalog No:112660 1:500) with HeLa cells lysate 1080ug.
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Background
MITD1 represents MIT, microtubule interacting and transport, domain containing 1
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References
- Lee S, Chang J, Renvoisé B, Tipirneni A, Yang S, Blackstone C. MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis. Molecular biology of the cell. 23(22):4347-61. 2012.
- Hadders MA, Agromayor M, Obita T. ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes. Proceedings of the National Academy of Sciences of the United States of America. 109(43):17424-9. 2012.
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