Cart 2

MIPEP antibody

Cat.#: 112628

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    MIPEP antibody
  • Documents
  • Description
    MIPEP Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, MCF7 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 70-80 kDa
  • Tested applications
    ELISA, IHC, WB
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    HMIP antibody; MIP antibody; MIPEP antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MIPEP recombinant protein (Accession Number: NM_005932). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112628(MIPEP antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112628(MIPEP antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112628(MIPEP antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112628(MIPEP antibody) at dilution of 1:50 (under 10x lens)

  • Background
    MIPEP(Mitochondrial intermediate peptidase) is also named as MIP and belongs to the peptidase M3 family. It performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. The enzyme is a monomer of 75 kDa with a transit peptide and about 20% of the fmal MIP preparation consists of equimolar amounts of two peptides of 47 kDa and 28 kDa, which are apparently the products of a single cleavage of the 75 kDa protein. MIP is an independent endopeptidase acting on mitochondrial intermediate proteins as substrates.(PMID:1322290).
  • References
    • Marcondes MF, Torquato RJ, Assis DM, Juliano MA, Hayashi MA, Oliveira V. Mitochondrial intermediate peptidase: expression in Escherichia coli and improvement of its enzymatic activity detection with FRET substrates. Biochemical and biophysical research communications. 391(1):123-8. 2010.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"