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Product Name
MIG6; ERRFI1 antibody
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Description
MIG6; ERRFI1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human tonsillitis tissue. Positive IF detected in PC-3 cells. Positive WB detected in HeLa cells, human brain tissue, mouse brain tissue, rat colon tissue, Recombinant protein. Observed molecular weight by Western-blot: 51 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ERRFI1 antibody; GENE 33 antibody; MIG 6 antibody; MIG6 antibody; RALT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MIG6; ERRFI1 recombinant protein (Accession Number: NM_018948). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112621(MIG6; ERRFI1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112621(MIG6; ERRFI1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:112621(MIG6; ERRFI1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:112621(MIG6; ERRFI1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The mitogen inducible gene 6 (MIG6) is located in human chromosome 1p36, a locus frequently associated with human lung cancer. MIG6 gene expression can be rapidly induced by factors including hormones, growth factor, and stresses. MIG6 encodes ERBB receptor feedback inhibitor 1 (ERRFI1) levels of which are very low in quiescent cells but upregulated by mitogens. MIG6 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.
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References
- Li Z, Dong Q, Wang Y, Qu L, Qiu X, Wang E. Downregulation of Mig-6 in nonsmall-cell lung cancer is associated with EGFR signaling. Molecular carcinogenesis. 51(7):522-34. 2012.
- Staal B, Williams BO, Beier F, Vande Woude GF, Zhang YW. Cartilage-specific deletion of Mig-6 results in osteoarthritis-like disorder with excessive articular chondrocyte proliferation. Proceedings of the National Academy of Sciences of the United States of America. 111(7):2590-5. 2014.
- Ajiro M, Jia R, Yang Y, Zhu J, Zheng ZM. A genome landscape of SRSF3-regulated splicing events and gene expression in human osteosarcoma U2OS cells. Nucleic acids research. 44(4):1854-70. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"