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Product Name
MIEN1 Polyclonal Antibody
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Description
Polyclonal antibody to MIEN1
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Tested applications
WB
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Species reactivity
Human
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Alternative names
MIEN1 antibody; C17orf37 antibody; C35 antibody; ORB3 antibody; RDX12 antibody; XTP4 antibody; migration and invasion enhancer 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human MIEN1 (NP_115715.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
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Validations
Western blot - MIEN1 Polyclonal Antibody
Western blot analysis of extracts of 293T cells, using MIEN1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"