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Product Name
Human Midkine recombinant protein
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Description
Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity).
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Protein name
Midkine
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Protein short names
NEGF2; ARAP; FLJ27379; MEK; MK; MIDKINE; NEURITE GROWTH-PROMOTING FACTOR 2; RP23-273F8.3; MDK
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Uniprot ID
P21741
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Gene Name
MDK; MK1; NEGF2
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human MDK (P21741) (Met 1-Asp 143) was expressed and purified.
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Protein Species
Human
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Molecular weight
The secreted recombinant human MDK consists of 123 amino acids and predicts a molecular mass of 13.4 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized human MDK (Cat:502729) at 10 μg/ml (100 μl/well) can bind mouse SDC4-Fc (Cat:503585) with a linear range of 0.16-1.25 μg/ml. -
Validations
Human Midkine / MDK Protein SDS-PAGE
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